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According to this, in the present work, the technique was applied to evaluate the discrimination between MRSA and MSSA strains ( Figure 1). Consequently, the technique captures the bacteria’s whole molecular fingerprint, which can be used to discriminate between bacteria at the genus, species and clonal levels. These results clearly demonstrate that EGCG exposure alters the expression patterns of key epigenetic and drug response genes with associated divergent-resistant profiles, which supports its potential for antimicrobial treatment and/or therapeutic adjuvant against antibiotic-resistant microorganisms.įTIR spectroscopy reflects the vibrations between atoms within molecules according to the atoms and bonds associated and from the interactions due to the atoms surrounding them in a highly sensitive and specific mode. Divergent resistant phenotypes were associated with divergent transcriptional expression of the epigenetic modulator OrfX, particularly in MRSA strains, as well as the key drug response modulators SpdC and WalKR. FTIR-spectroscopy analysis enabled the clear discrimination of MRSA/MSSA strains and the EGCG exposure effect in the bacteria’s molecular profiles. The transcriptional expression of OrfX, SpdC and WalKR was performed by using qRT-PCR.

The bacteria’s molecular profile was assessed through FTIR spectroscopy. The antibiotic synergistic assay was performed through disk diffusion according to EUCAST guidelines with co-exposure to EGCG (250, 100, 50 and 25 µg/mL). aureus resistance phenotype was performed through automatized methods. aureus strains ( n = 32) were isolated from infected patients in a Lisbon hospital. EGCG exposure effects in epigenetic and drug resistance key modulators were also evaluated.

aureus strains with divergent resistance phenotypes. We aimed to assess the antimicrobial potential and synergistic effect between Epigalocatenin-3-gallate (EGCG) and different antibiotics in S. Healthcare-associated methicillin-resistant Staphylococcus aureus infections represent extremely high morbidity and mortality rates worldwide.

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